Witec alpha 300r confocal raman microscope user manual

The imaging capabilities of the alpha300 r fulfill the highest requirements of confocal raman. The alpha 300r plus from witec is a confocal raman microscope with two scanning systems, a coarse motorized stage for large areas and a piezo stage for fine, sub nanometer adjustments. Some papers mentioned that the pl measurement was carried out using a witec alpha 300r confocal raman system, but they didnt mention the principle of it. The alpha300 r is witecs preeminent confocal raman imaging system, setting the benchmark in terms of flexibility, sensitivity, speed and performance. Witec atomic force microscopes are developed and designed to allow combination with other imaging techniques such as confocal raman imaging. The confocal raman imaging system alpha300 r from witec offers the unique ability to acquire chemical information nondestructively with a resolution down to the optical diffraction limit 200 nm. Confocal raman imaging microscope witec alpha300 r raman microscope featureshigh speed, high resolution confocal raman. The witec raman is an advanced confocal raman microscope that combines fast. The witec confocal raman imaging system alpha300 r offers exceptional performance in speed, sensitivity, and resolution. Identification of microplastics using raman spectroscopy. Witec alpha 300r quick operation summary october 2018.

The witec raman is an advanced confocal raman microscope that combines fast imaging, high spectral sensitivity and spatial resolution with excellent analysis software into a system designed around imaging. The dxr2 smartraman spectrometer, dxr2 raman microscope, and dxr2xi raman imaging microscope, in their default configurations, are class 1 laser products. Witec alpha 300r confocal raman imaging system has the ability to acquire chemical information nondestructively with a high resolution 200 nm. Snom, confocal microscopy and afm system witec quantum. For information about all other features, the user is referred to the witec project manual. It is a scientific instrument allowing stateoftheart, spatially and temporally highly resolved raman.

Confocal raman imaging witec alpha 300 coupled with atomic force microscope. The witec atomic force microscope alpha300 a is a reliable, highquality nano. Confocal raman operation manual university of minnesota. Serial section raman tomography with 10 times higher depth. Witec confocal raman microscope alpha300r nanoscience. The system is equipped with 532 nm green laser, max. An automated particle analysis tool combined with confocal raman microscopy provides a convenient solution to the challenges of microparticle analysis.

Window should be adjusted see witec control manual section 3. Only these functionalities and their corresponding spectroscopy options are described inthismanual. Confocal raman microscopes from witec raman, afm and snom allinone system. Confocal raman microscopes from witec superior confocal raman imaging system. Microscopy center ufpr transmission electron microscope, jeol jem 1200, which operates with a voltage of 120 kv. The alpha300 s is a user friendly scanning nearfield optical microscope snom that combines the advantages of snom, confocal microscopy and atomic force microscopy afm in a single. Confocal raman imaging microscopy is not a pressonebutton technique for hasty end users. This document specifies the work instructions for the cmi witec confocal raman microscope located in the ground floor physiology lab, quadrangle building. Witec alpha 300 confocal raman microscope is a highquality raman imaging instrument that allows the researchersscientist to explore high spatial resolution imaging of the sample. Confocal raman microscope allows one to do high resolution chemical mapping on materials in 2d and even 3d. Our confocal raman imaging technology leads the industry in speed, sensitivity and resolution. Confocal raman imaging microscope for 3d raman and ultrafast raman imaging. The confocal raman imaging system offers the unique ability to acquire chemical information nondestructively with a resolution down to the optical diffraction limit 200 nm. The system has high confocality and boasts a minimum depth slice of 350 nm.

Confocal raman microscope center for materials, devices. The objective in position to start with should be 10x. The atomic force microscope witec alpha 300 ais a modular, user friendly atomic force microscope. This allows to observe and analyze the distribution of different phases within a sample in ambient conditions without specialized sample preparation.

Chemical imaging of drug delivery systems with structured. Superior confocal raman imaging system witec quantum design. The alpha300 r represents a new generation of raman imaging systems, focusing. Witec s solution for correlative raman sem imaging is now available for zeiss sigma 300, a field emission scanning electron microscope fesem. The witec alpha300 ra correlative microscope fuses the most powerful chemical and topographic imaging techniques into one fullyintegrated instrument. The witec alpha 300r plus confocal raman microscope.

Scanning nearfield optical microscopes snom from witec combined raman and scanning nearfield optical microscopy snom system. The witec alpha 300r plus confocal raman microscope youtube. Raman imaging generates hyperspectral images with the information of a complete raman spectrum at every image pixel. This enables the user to take 3d raman images with submicron. Ftir agilent spectrometer model 670ir combined with the microscope 620ir. Confocal raman microscope with unrivaled resolution and sensitivity. Confocal raman microscopy is an analytical technique with a steadily increasing impact in the field of pharmaceutics as the instrumental setup allows for nondestructive visualization of component distribution within drug delivery systems.

For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman imaging in combination with scanning nearfield optical microscopy for optical imaging with resolution beyond the diffraction limit. Confocal raman microscope with unrivaled resolution and sensitivity the alpha300 rrepresents a new generation of raman imaging. Witec alpha 300 rs witec raman microscope combined. Atomic force microscopes for research combined microscopes confocal raman microscopes filters. Witec s alpha 500 combines confocal raman imaging and atomic form microscopy into a fully automated and integrated system for scanning multiple samples andor large sample areas. Imaging of sections was conducted with a confocal raman microscope witec alpha 300. The alpha300 m is a highquality yet affordable micro raman system that provides the same outstanding performance and spectral resolution that other witec. This is available for any collaboration with other research. Raman, afm and snom allinone system witec quantum design.

Witec microscopes in conjunction with the alphacontrol. Its easy to use with great software to perform postdata analysis. Access raman data faster than ever with this easytouse pointandshoot raman microscope. It combines all features of the alpha300 s and alpha300 r and many afm operation modes. Witec apyron imaging system has been developed to overcome the boundary between easeofuse and ultimate capability in raman imaging. Witec is a leading german manufacturer of confocal and scanningprobe microscopes for stateoftheart raman, atomic force microscopy afm, scanning nearfield optical microscopy snom and developed the integrated raman imaging and scanning electron rise microscopy. At this juncture may 2018, a search on isi web of knowledge, researchgate and sciencedirect yields a total of 71 original articles on mp identification using raman spectroscopy. The alpha 300r plus confocal raman microscope from witec. Cmi standard operating procedure confocal raman microscope. Advanced imaging capabilities make the new thermo scientific dxr3 raman microscope faster and more reliable than ever.

Combined raman and scanning nearfield optical microscopy. Witecs rise microscopy now available with zeiss sigma 300. For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman. For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman imaging in combination with scanning nearfield optical microscopy. The confocal raman imaging system offers the unique. The alpha300 r is witec s preeminent confocal raman imaging system, setting the benchmark in terms of flexibility, sensitivity, speed and performance. For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman imaging in combination with scanning nearfield optical microscopy for optical imaging with. If you see an area where more clarification is needed, if additional information is needed, or if you have suggestions on how to make this guide more useful in the lab, please contact. Confocal raman microscopy witec alpha 300r center for. The confocal raman imaging system alpha300 r offers the unique ability to acquire chemical information nondestructively with a resolution down to the optical diffraction limit 200 nm. Confocal raman microscopy witec alpha 300r please refer to witec alpha300r confocal raman microscope user manual for the details of the operating procedure. It allows hyperspectral image generation with the information of complete raman. Witec alpha300r confocal raman microscope enables confocal raman imaging. Witec alpha 300r quick operation summaryoctober 2018.